Force measurements

Page under construction

Stand: Zeiss Axiovert 200M (inverted)

Scanhead: Galvo Scanner

Illumination: laser: 405/488/555/639 nm

Lamp/LED: Metal Halide

Detection: Multi Alkali PMTs

xyz: motorized

To be equipped with AFM (JPK Nanowizard 4)

Samples: fixed (and live), rather not suited for sensitive samples

- Atomic resolution

- Fast scan mode (> 630 lines / s, > 10 fps)

- Manual stage and motorized hybrid stage

- Petri dish heater

- CO2 incubation

- E.g. microrheology, force spectroscopy, imaging

- Compatible with Zeiss LSM 700 -> combined AFM / LSM experiments

Combined with an inverted optical microscope with confocal laser scanning and transmitted light

Equipped with petri dish heater and an optional hybrid stage for extended z-ranges (e.g. for cells).
Contact-, Tapping-, force modulation-, and easy imaging modes: "PeakForce Tapping" and "QI-Imaging"
Force spectroscopy/molecular manipulation.
XYZ Tip-scanning in air and fluids
100x100x15 μm^3 scan range
Fast scanning option up to 150 lines/sec with a 100μm scan range
Transmitted light microscopy contrast methods (e.g. bright field, phase contrast, Hoffman modulation or DIC) possible simultaneous to AFM imaging
confocal laser scanning fluorescence microscopy possible simultaneous to AFM imaging
"direct overlay" option for precise alignment of AFM and optical images
temperature control for fluid samples
data processing software

The inverted confocal laser scanning microscope is a Zeiss LSM700 with a motorized xy stage.

2 PMT channels and a transmission detector

Laser Lines: 457/488/514/543/633

Bruker JPK NanoWizard 4XP AFM