Force measurements

Applications:

• Topography imaging, micro-rheology
• Force spectroscopy
• Molecular manipulation

In combination with a Zeiss LSM900 confocal fluorescence microscope

Stand: Zeiss Observer 7 (inverted)
Scanhead: Galvo Scanner
Illumination: laser: 405, 488, 561, 639 nm
Lamp/LED: 
Detection: GaAsP PMTs
Samples: fixed (and live), rather not suited for sensitive samples

- Atomic resolution

- Fast scan mode (> 630 lines / s, > 10 fps)

- Manual stage and motorized hybrid stage

- Petri dish heater

- CO2 incubation

- E.g. microrheology, force spectroscopy, imaging

- Compatible with Zeiss LSM 700 -> combined AFM / LSM experiments

Combined with an inverted optical microscope with confocal laser scanning and transmitted light

• Equipped with petri dish heater and an optional hybrid stage for extended z-ranges (e.g. for cells).
• Contact-, Tapping-, force modulation-, and easy imaging modes: "PeakForce Tapping" and "QI-Imaging"
• Force spectroscopy/molecular manipulation.
• XYZ Tip-scanning in air and fluids
• 100x100x15 μm^3 scan range
• Fast scanning option up to 150 lines/sec with a 100μm scan range
• Transmitted light microscopy contrast methods (e.g. bright field, phase contrast, Hoffman modulation or DIC) possible simultaneous to AFM imaging
• confocal laser scanning fluorescence microscopy possible simultaneous to AFM imaging
• "direct overlay" option for precise alignment of AFM and optical images
• temperature control for fluid samples
• data processing software

The inverted confocal laser scanning microscope is a Zeiss LSM700 with a motorized xy stage.

2 PMT channels and a transmission detector

Laser Lines: 457/488/514/543/633

• LUMICKS Generation 2 dual-trap optical tweezers
• fast detectors
• 3-color confocal laser scanning for simultaneous fluorescence measurement
• temperature control